Molecular Signature and Anataomical Features of a Hypertrophic Scar From Abdominal Skin of a 36-Year-Old Female
Hypertrophic scarring is formed as a result of abnormal wound healing. Efforts have been made to understand the mechanism of hypertrophic scarring for prevention and treatment, mainly by using several model systems. However, animal models do not form effectively hypertrophic scars. Desorption electrospray ionization mass spectrometry imaging enabled label-free elucidation of the spatial distribution of metabolites and lipids in skin tissue samples under ambient conditions of a 36-year-old female. It revealed up-regulation of phospholipid sphingomyelin SM(18:0/16:1), m/z 235.18, and metabolite sphingosine, m/z 310.24, which resulted in skin hardness and defects in the skin barrier function. The scar tissue also showed downregulation of phospholipid cardiolipin CL(62:2), m/z 1322.03 that indicated defects in autophagy function.